irshadaad Posted August 9, 2009 Share Posted August 9, 2009 what s the proper method for thawing FFP Link to comment Share on other sites More sharing options...
RR1 Posted August 9, 2009 Share Posted August 9, 2009 Hi irshadaad, there are various ways of thawing FFP- depending on the equipment you have available. How do you currently perform this? Link to comment Share on other sites More sharing options...
jfboyer Posted August 9, 2009 Share Posted August 9, 2009 Hi Irshadaad, we currently use a 37 degree C waterbath. We take the units from a -40 degree C freezer, do our computer work with the units, place the units into a plastic bag to protect the ports from any possible contamination of the water (which would be minimal anyways) and place the units at least 3/4 submerged into the bach for around 20 mins. (depending on how large the units is and to make sure they it is completely thawed.) Hope this helps. Link to comment Share on other sites More sharing options...
eric1980 Posted August 17, 2009 Share Posted August 17, 2009 This is what we do for years. But I cannot find in my lab on what is the basis we chose this method.If you need speed, there's this thawer which uses microwave. There will be no wet work (literally), and it takes 5 minutes. But I really do have concerns on the viability of the coagulation factors after using it. Link to comment Share on other sites More sharing options...
Judy hedglin Posted August 27, 2009 Share Posted August 27, 2009 At my facility, we thaw FFP for 15-30 minutes at 30-37 degrees. If temp reach over 37, the heat labile factors can be destroyed. Link to comment Share on other sites More sharing options...
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