monitoring weak reaction on Ortho ProVue

[apugh]

Hi everyone, we recently went live with our second Ortho ProVue. We had a patient test negative for the antibody screen on our initial ProVue. It just so happened that a week later that same patient was run on the new ProVue and the screen was weakly positive. An Anti-K was Id'd. The most recent specimen was then run on the older ProVue with a negative result. The initial patient specimen was then run on the new ProVue with a weak positive result. Ortho was called and service was dispatched. The camera was realigned, and other things were checked too. We then ran a very weak specimen on both ProVues with the same weakly positive result. So, for now both ProVues seem to be picking up weak reactions. What we would like to know is if anyone has experienced this same incident. Also, does anyone monitor or QC weak reactions on the ProVue. We use the Alba controls, but they are quite strong. I'm hoping someone out there has some ideas. They would be greatly appreciated.

Thanks!

[KECIA]

Were your screening cells the same lot. Was the K expression **** or hetro did you look at that as a reason?

[apugh]

The Lot # of reagent red cells and gel cards were the same. The K expression is usually always hetero. We are concerned about the camera drifting off again and this situation could happen again.