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  1. We have recently switched from using the Ortho ProVue to the BioRad tango Optimo. With this, we have encountered some issues. The tango does not pick up Du on the routine ABO/Rh strip. The ProVue ABO/Rh card does. So, we have had a lot of discrepant Rh types since we have started using the tango. We use the Quotient D Blend as our serologic reagent, which does pick up Du at immediate spin, as well as Du & D6 at IAT. In the last week we have had two OB patients type as Rh negative both on the tango and at immediate spin with the Quotient D blend. On one patient, the nurse called and stated that the patient had a history of being Rh Positive in their prenatal workup that was done at another lab. On the other patient, their Rh type was not questioned until their Fetal Bleed Screen was performed and was macroscopically positive. Both were testing through IAT with the Quotient D blend and with the Ortho ABO/Rh gel card (manual method) and were found to be Rh positive. I feel like when we run into these patients with discrepant types, we are chasing our tails testing by 3 methods to confirm their Rh type and am wondering if there is a better way/process? What does your facility do for routine D testing? What about Du testing? We typically only perform Du testing on Rh negative babies born to Rh negative mothers and of course when an investigation is needed like the above situations. Any wisdom is great appreciated! TIA!
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