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Showing results for tags 'liss'.
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Hi all, I'm still a relatively new blood banker and was wondering if you all would be willing to shed some light on the different methods of blood banking? We currently use solid phase but will be switching to gel next year. I have some questions regarding each method but also wanted to see what seasoned techs have experienced with them. Solid-phase: Incubation @ 37 with potentiator Wash AHG Read Pros: Sensitive Cons: Question: Won't pick up on clinically insignificant cold autos because of less exposure to cold temperature during the process? Or is it due to something else? Good to use with insignificant cold auto if you don't want to pick them up on screen? ______ Gel: Incubation @ 37 on top chamber, with potentiator? No washing Spinning down through the matrix containing AHG Pros: Very sensitive Cons: Will pick up insignificant colds due to the chamber temperature and matrix can catch IgM? Rouleaux Notes: IgM remains as there's no washing _______ LISS Tube (skipping IS phase): Incubation at 37 with LISS Reading (for strong IgM or IgG) Wash (removing unbound Abs) Add AHG Read (for IgG) Pros: Not too sensitive, not too weak Cons: Won't pick up weak antibodies Question: Won't pick up insignificant warm because LISS isn't strong enough? (Such as weak warm auto) Good to use when not wanting to pick up warm auto on screen? ________ PEG Tube: Read at IS (for IgM) Add PEG, incubate at 37 (30min inc. might pick up insignificant Abs?) Wash Add AHG Read (for IgG) Pros: Strong than LISS Cons: Please feel free to share any thoughts or experience.
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- gel antibody screen
- solid phase
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Hi everyone, Does anyone have procedures to give helpful hints or other source to know when it is best to use Peg, LISS or anyother potenatoirs? Some antibodies are better with others and want a good cheetsheet to have in place to help, especially since we just introducing Peg. Do you use Peg as 1st option when needing tube? Peg cant be read at 37 so if want to know if antibody is reacting at 37 need to use LISS. Anymore to add??? Thank you!