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May 2024 Challenge

Emwilson7

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Everything posted by Emwilson7

  1. Emwilson7
    " Please explain better. What identification will be possible?
  2. Emwilson7
    Exactly what Malcolm said about concentrations. When you treated the 2 sets of cells, did you follow the exact same procedure for both? (incubation time, temp, amount of DTT solution added, same concentration of DTT, number of washes, etc.) If there was any differences between the 2 sets, maybe that could account for the kell antigens not being destroyed. Try treating a larger aliquot of those cells at once, splitting them into 2 sets of tubes after treatment, then run one set against your anti-k reagent and the other against your patient anti-k. If your k antisera is still negative, and the patient anti-k is still positive, then your treatment works fine and you'll know there's some other antibody contaminate in your patient anti-k sample.
  3. Emwilson7
    When you did your first treatment on your 3 cell screen and your testing with k antisera came out negative, did you run the known patient anti-k on the same cells or on a separate 2nd treatment of those cells? If you did a second treatment on a different aliquot of cells, then ran the patient anti-k on those, it may be possible you didn't treat them long enough to completely denature the anitgens. It could also be possible that your patient with the known anti-k also has another antibody whose corresponding antigen is not affected by DTT, and that's the remaining weak reactivity.
  4. Emwilson7
    I've already got something scheduled for those days in February unfortunately. I already got the SBB exam prep materials from my coworker who went to AABB this past year. It's got a great section for review of math calculations, which I REALLY needed. I didn't realize how much of it I had forgotten. Thanks everyone for your input!
  5. Emwilson7
    Perfect, thanks so much! I appreciate it
  6. Emwilson7
    Thanks! I've read the technical manual cover to cover, been through Dr.Garrattys transfusion medicine lectures for SBB students, and gone through all the review materials my coworker brought back from AABB. In combination with my work experience as a reference tech for the past 3 years, I'm hoping that's enough, but when I go through practice questions I still don't feel so confident.
  7. Emwilson7
    Has anyone taken the SBB exam in the past year? I've been putting it off, and I finally registered to take it sometime in the next 2 months. Any advice?
  8. Emwilson7
    The paper I linked has Dr.Garraty as the author of the third part. Maybe it's the one you're thinking of? Unless you're thinking of a newer paper, that one is from 2002.
  9. Emwilson7
    I didn't get to go to AABB this year unfortunately, but I found a paper that may help! http://asheducationbook.hematologylibrary.org/content/2002/1/445.full.pdf
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