[Ortho panel C ficin issue]

yes we have seen this on several occassion and reported to Ortho. They gave explanation to the ref. in IFU:

Antibodies that require Anti-IgG to react will not be detected by this technique.

I've scanned the fine print of the IFU, REF 6902319 (0.8% Resolve Panel C System/Untreated/Ficin Treated) several times and can't find the above quoted statement in the insert. Do you mind sending that over the thread for my perusal?

Much appreciated, aakupaku

[Ortho panel C ficin issue]

Just curious if those who are runnung the ficin panel with IgG cards are doing so manually, or if you've figured out a way to run them on Provue.

thanks!

[Ortho panel C ficin issue]

Thank you for the responses.

And thanks for confirming our findings, aakupaku!

BB Bryan

[Ortho panel C ficin issue]

We've noticed that Kidd antibodies are not being enhanced by the Ortho ficin treated panel cells (multiple lot #s of panels and gel cards). For weak Kidds, the untreated panels are often reactive, while the ficin treated panel is nonreactive. Ortho's package insert recommends using buffer gel cards with the ficin treated cells (Provue will only perform the ficin panel with buffer cards loaded).

After further investigation, we've discovered that performing the ficin panel with IgG gel cards (manually) provides the expected enhanced Kidd antibody reactions. Bottom line, buffer cards are not working according to the manufacturer for Kidd antibodies. Whether the Kidd Ab IgG component structure isn't buffer gel sensitive, Ortho won't speculate. Nor has Ortho admitted to any other record of this occurance.

Has anyone else noticed this phenomenon? Beware

[Helmer UltraCW cell washer ???]

I am a beta site for Helmer for a non bleach product - microcide. After using Sorval cell washers for 35 years at multiple institutions and never bleaching the systems and never having a problem I have recommended to the manufacturer monthly cleaning with microcide. is adequate. Tesiting is not yet complete. Stay tuned.

Any update on the PM schedule with Microcide from Helmer? It would be great to have something official as I'm currently writing the SOP. THX!

[Hettich EBA 21 Serologic RPM setting]

The Helmer rep was completely clueless as to recommended RPMs for serologic testing (ABORH, etc.).

I've tried using an online g-force calculator and came up with ~3012 RPM (based on head radius and what seems to have been the g-force of the Dade Immufuge II (39.8 N, based on 90mm and 3175 RPM).

3010 RPM seems a little low to me. I've read other folks being set up at 3300.

Does anyone know the proper RPM setting for sero testing using the SO head?

Muchas Gracias!