[sorvall LEGEND RT]

GOOD evening , 

please any one using Sorvall Legent RT bench top for making blood component , that is from whole blood to platelet and FFP .

i need to know the setting of machine like speed , time and temperature for 1st run ( whole blood ) to get PRP and 2nd Run to separate platelet and FFP  .

any additional information will be appreciated.

many thanks  

[Use of wash RBC´s]

We have one Thalassemia major patient who has multiple antibodies . we used to Washed the Blood Bag three times and add little saline . ( after finding compatilbe and all antigen nagative blood for the patient ) . I don't have any documentation or recommendation for it . just to share which may give some idea by some one here. 

[Ortho ProVue "?" reactions]

yes we are doing this modifying result by visual inspection . some time we confirm it by manual or repeat method 

[Daily Quality Control]

we do QC on every start of the shift (8 hour), or as required like new reagent .

[Vitros 350 (ortho clinical diagnostic)]

thanks Cliff , 

 

yes some guide line for Validation and Record of the machine . any documents plz which give us the idea to go through

[Vitros 350 (ortho clinical diagnostic)]

Good Morning every one.

We are going to start with vitros 350 chemistry analyzer , i would like any kind of help regarding documentation and validation .

Or any guide line which hepls us and make us comfortable .

thanks in Advance

Regards 

Changezi 

[Am i missing some thing ? need help]

THANKS for reply , yes i we are using the same ortho reagent , and the preservative is same for both , today i  checked with the freash sample from the patient post op , i find it weaker reaction like 0.5 , 0.5 , 1 respectively with ortho and now manuall its shows negative now still i am not clear with that .

 

thanks David for your contribution

[Am i missing some thing ? need help]

Good Morning every one , i am just dealing with one patient

BLOOD GROUP    = A POSITIVE

AUTO                     =NEG

DAT                       = NEG

XM                          = compatible (with all 3 unit including one O+ unit)

IAT        l = 2+ , ll = 2+ , lll = 3+

ABI panal A (Identification)  = NEG

 

repeated with change screening cell but result is same .

 

a lots of thanks in advance

 

 

anyway we are using ortho Autovue Innova

[bbguy.org site]

A vary nice presentation and lecture i like how he guide about ABI and the discussing diff cases

[Antibodies Identified at Another Facility]

we put ABI result in our computer system which will blink at the time of entering XM and where we must inter for the DONOR that the donor is negative for that Antigen .

 

for example Anti-K is present , so at the time entering XM for that patient we must enter Negative FOR KELL (our code is NKEL) with the donor nubmer other wise it will not accept the compatibiity .

[Automation in the Transfusion Lab - what do you use and do you recommend it?]

Using Ortho Clinical Diagnostics AutoVue Innova.
 

[How would you have handled this?]

^{you should call the floor and stop the transfusion or hold the transfusion ,}

 

^{2nd we have a policy that as you recived the O-neg  from  our supplier we always rechecked the group and also for week D.}

 

^{as we are working in trauma centre and many time we send O neg with out any test or just on IS , so our all O neg in out stock is Already checked .}

[Me, when I was VERY young, before I became a serologist!]

wow thats a piece of trasure for you , any way you canbe a Fire safety officer of you lab now.

[test upload]

hay hi , from long time i want to ask to know , in previous website i was always going through the "WHATS NEW"

 

it helps me to see whts new topic or discussion , now i feel i miss some topic i saw these topic vary late .

 

or guide me how to go that not to miss any new topic started ,

 

( i am not good with the computer too much , your help whould be appriciated)

 

Changezi

[:)]

HI ,;

[Which is more suitable A neg or B neg RCC's for an AB neg patient?]

American Red Cross has a chart in their blood transfusion guidelines that states that the second choice PRBC for an AB patient would be A and the third choice B. Hope that helps.

And the same above 1st choice A and then B , it is mantioned in AABB

( chapter 20# Hemotherapy Decisions and Their Outcome)

(Table 20-4 , page 576 , 16th edition)

And more is Malcolm Needs already explained

[Ruling out when HTLA present]

I notice that the patient is female. If she is of "child-bearing potential" (as I've said before, a hateful phrase, but in vogue) then I would give her K- blood anyway, on the grounds that she is obviously a "responder" and you don't want her to make anti-K, as this is a pretty nasty antibody in pregnancy. If she is beyond "child-bearing potential" (a phrase that makes you sound like you are talking about cattle!), then it is not so important.

Agreed with Malcolm , for female with child -bearing age we alway use K- blood

[Accepting RH type results on OB patients from other facilities]

I REMEMBER we discuse the same matter on FFP transfusion on previous record .

and most of us agreed on having repeat the group on every addmission once the patient leave the hospital . so when we are Rechecking on our own record how possible to beleave on other hospital record , so many cases discuss on that thread .

So always better to repeat the group+screening on each addmission .

my question is WHY to take the Risk ... ?

[Saline QC]

well we are now reciving comercially prepared saline , but for manual i just check with RBC make the suspension centrifuge and check for the hemolysis ,

(just forget about my english always for my all post :juggle:

[Ideas and interpretation on Emergency Release forms]

HI ALL , we have a emergency form, which is there in emergency ,the porter came directly with form to take the blood and is called

Emergency blood release form

1 = O neg without screening Un-Xmatch blood

2 = O neg screen blood Un-Xmatched

3 = Group to Group with immidiat spin

no of blood required ______

signed DR xyz

(P.s in case of more blood required we go for O+, depend on the stock )

but if the number of patient is more we do all documentation later after the emergency finished , because the situvation is totally diffrent when the number of patient becomve more like 10 or 20 or more than this its depend , but for routien emergency this form is okay its simple just one tick and signe and got the blood

[how to prepare check cells ( coomb's control cells)?]

Dear ahmed,

I was wondering if you could send me your protocol with details for me.

Thanks

did you mean SOP you need ??

[how to prepare check cells ( coomb's control cells)?]

make the wash O+ cell suspension add a drop of Anti-D , incubate for 30min and ready to use

(put the prepration date ... weekly better)

[Hi]

WELL COME jamie , ya you are right we learne here alot i visit here every day once atleast its wonderfull , so many experience persons are here like mabel and malcolm needs , cliff , dr paper , liz and so on....

you will find the people here who's passion is blood banking

[Reading DAT microscopically]

we using gel , and if we found some MF or not a cleared result like a vary weak reaction with our automation shows "?" mark .

then we confirm it with tube method and finalised it with microscopy by running along control or check cell .

[Transfusing A2 patients]

we directly go for group 0 , as we find anti-A in patient . happy staff, happy computer, happy time too