Bb_in_the_rain

Hello, Great discussion. I just set up a comparison study using DTT made with different pH buffer and here is the data (a copy of poster to be posted at 2017 AABB meeting). Hope it help answering some questions here. to print DTT poster AABB 2017.pdf

Hello, We have frozen rare cells in glycerol in this lab. Whenever we use these cells, we have to thaw the entire vial, take out some cells and refreeze it (where in LN2 frozen cells never needed to be thawed once frozen). I always wonder what happen to those cells frozen in glycerol once they goes through about 3 cycles of freezing and thawing. I had thrown out a few vials because the cells are all hemolyzed. But I do not think it is because of the age of the cells since I had successfully thawed a few sources that are about 15+ years old. Any thoughts ?

20 months old patient with respiratory problem. Patient is O+ and antibody screen is negative by gel method at the hospital. Lectin panel was ordered at our lab. Here is our results. Arachis Hypogea - Patient's cells 4+, Neuraminidase treated cells 4+, Pooled O cells negative Glycine Soja - Patient's cells neg, Ficin treated cells 4+, Pooled O cells negative Dolichos biflorus- Patient's cells neg, A1 cells 4+, pooled O cells negative Salvia Sclarea- Patients cells neg, ficin treated A cells 2+, pooled O cells negative Pooled adult AB plasma- Patient's cells neg, Neuraminidase treated cells 4+, Pooled O cells negative: test with 6 more sources of AB pooled plasma, all negative with patient's cells and positive with neuraminidase treated cells. Pooled cord AB plasma- Patient's cells neg, pooled O cells negative. Based on above reaction, I can assume this patient has weak form of T polyagglutination, Tk, Th or Tx but I do not know why patient's cells are not reactive with adult plasma. Any thought? Should I incubate at room temp longer ?

I think it depends on the method used. ECHO solid phase has shown to have weak to negative reactions with screen cells and panel cells from what I have seen so far. But non-the-less they were positive screens (weakly reactive but not all cells were reactive) that we had to work up an antibody identification. By tube method. it is reactive with PeG, LISS and saline IAT.

I am guessing red cells doesnt get destroyed because CD38 expression on red cells are very low?

This antibody will not be adsorbed out since the dose is very high relative to CD38 expression on RBC.

I have recently done an oral presentation about our research project on anti-CD38(Daratumumab) during this recent AABB meeting in Anaheim. I have some abstract references that I have attached here. Hope it helps. Also a full article here. http://onlinelibrary.wiley.com/doi/10.1111/trf.13069/full 3 of 8 patients(i have seen to date) has positive DAT and 2 of them were on IVIG. We found that this antibody is non-reactive with In(Lu) cells by tube method. By tube method, DTT treated cells were non reactive, cord cells were weakly reactive to non-reactive.(not enough cord cells were tested yet to find pattern) Our flow cytometry data wiith florusecnt labeled anti-CD38 shows that DTT treated cells has very low percentage of CD38 (2%-4%), cord cells has about 21%-24% and positive control varies from (53-82%), In(Lu) cells has about >20%. These data were presented orally during AABB meeting but not yet been published. SKMBT_75115100213120.pdf NEJM Aug 2015 editorial on Dara.pdf cord rule out.pdf abstract-ARC and NYBC.pdf abstract-ARC and NYBC.pdf

Do you consider readjusting pH of blood bank saline using NaOH?

I believe this book is no longer available anywhere for purchase. Any chance that the book can be requested for reprint from the publisher? I believe there were several reprints on 3rd edition but not the latest edition (4th edition). Is this publisher (Montgomery Scientific Publication) still in business? Anybody know?

Unfortunately our lab switched our Anti-Jka reagent that it is no longer available for me to play with

I found this on the internet. Please let us know the outcome if you look into this. http://eylabs.com/product/anti-n-vicia-graminea-lectin-vga-2ml/?distributor=true#.VlUmFexVhBc

Yesterday, I found out from a senior tech that weak positive (rough reaction) were often found with this Ortho Monoclonal reagent (I will look up the clone number when I get to work today). in 1995 (way before I started working in this lab) when molecular genotyping was being validated here, most of these units that has weak reaction are Jk(a-) molecular typing and variant Jka was not found when sequencing was done. Anybody else having these "rough" reactions with Ortho Monoclonal Anti-Jka? Should I stay away from this reagent when performing donor typing? Or should I keep using this reagent to type donors and avoid labeling units with "rough" reaction as Jk(a-)? Any thought?

I do not have much knowledge about these variants. Thank you very much for the lecture slide and helpful advice. I am hesitating very much to enter this unit as Jka-, though this donor has been typed as Jk(a-) three times since 2011. Thank you very much for the assurance. P.S. Love slide number 38

I typed a unit of RBC with different sources of anti sera and the following are the results. 1) Ortho monoclonal Anti-Jka (manufacture insert says incubate at room temp 5min to 30 min and read macro) at 5' incubation- 0 at 15' incubation- w repeated result at 5 min incubation - 0 at 15 min incubation - +/- or micro (even though insert says to read macro, I read micro because it seems "grainy" on the mirror) 2) Immunocor IAT reagent types negative 3) Unit will be typed with Bio Rad Monoclonal reagent today but I think I am going to type negative with this reagent since it react weaker. My question- is this unit Jka neg, Jka pos or maybe Jka variant? As Ortho reagent is monoclonal, should I be expecting other specificities in this antisera bottle? I learned that monoclonal reagent are very specific? Above all, is it safe to give this unit to patient with Anti-Jka? Thanks in advance for your replies.

I am just typing my thoughts outloud (not that I have seen anything like that during my very short career) Maybe soluable A or B substances from non O recipient's serum adsorbed onto O BM donor cells after engraftment? Case #2 can some chemicals or antibiotics in monoclonal reagent cause positive reaction? that does not explain the reaction only with unwashed cells. Maybe something is coating on patient's cells? Perhaps this patient has too much protein floating around in its plasma and they got onto the cells?

I am taking the exam in january 2015. I dont think I will be able to make it to the review session. Is there anyway that I can get the materials?

Huge Congrats on Passing!! I am taking it this January and very nervous about it. Were there any molecular question regarding blood groups in your exam, Single neucleotide Polymorphorisms etc... I saw alot of those in MNS blood group system when I was studying but cannot remember these amino acids for my life. Since I am taking mine in January, I will not be able to make it to Gulf Coast Review. I attended AABB and found their notes to be very helpful. Is there a way that I can look at the notes from Gulf Coast Review (to see if I am not missing anything in my studying)?

I am planning to take it this January. Would like to join if there is any study group going on. I just came back from 2014 AABB meeting with their review notes and they are very helpful! I am planning to take it this January. Would like to join if there is any study group going on. I just came back from 2014 AABB meeting with their review notes. Watched some Youtube Videos on Immunology, Coag etc and reading AABB manual, Blood Group Antigen Facts Book, Blood Transfustion Therapy-A physician's handbook. Browse through Harmenings Clinical Hematology Book and Daniel's Blood group book (for locations and interactions). I heard that review from Gulf Coast Reginal MC is very helpful but I dont think I will be able to make it since I am taking the test in January. Anybody taking it around the same time as I am?

I am located in California and work in a transfusion service with a big population of stem cells transplants. What captured my interest is that I had an Rhpos patient receiving Rh neg HPC and developed Anti-D right before blood type changed to Rh neg. I found out that passenger lymphocytes response against patient's red cells while engrafting. I also found a couple of articles about rh neg pt receiving rh pos hpc that does not produce anti D due to chemo. My interest is to look through Rh pos pt that received rh neg HPC to see how many has produced anti D. We also has patients producing warm auto and other allo antibodies post transplant despites immunosuppressant and chemo that they are receiving. Makes me wonder why...

I haven't done any publications before. If I see some interesting cases and would like to write about it, what are my options?