The Sysmex XN-Series multiparameter automated hematology analyzer, consist of a new channel named the WDF channel. This channel can differentiate leukocytes from cells treated with specific reagents containing detergents and fluorescent stains, by using the 2-parameter flowcytometric method. The scattergrams of the 2 channels have different patterns due to the differences in the reagents used as well as differences in the hardware and software. In particular, the WDF channel differentiates between lymphocytes and monocytes and enhances the separation capacity.
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