Hi all, my first post on here. I'm no expert in blood bank issues but am very keen and would like to find some info about the following, if anyone can help I will be very gratefull. Sorry if the questions are fairly basic to some of you. 1. Cold aggs and the best way to deal with them? - Warm the sample and carry out the group and antibody screen using warmed equipment - pipette tips, cards etc. If there is no antibodies present the screen should be neg. If the screen is positive could this be due to the prescence of an antibody or the cold aggs? and how would you proceed? Where does the keeping the patient sample at 4C for an hour come into play, if at all?? 2. When identifying antibodies via a panel, why is it that the coombs panel will, most of the time, be clear cut and show easily a Kell, E, C etc but when you do an enzyme panel the panel results just dont match up to what you were expecting? Is it because the panel is also picking up non specific reactions as well as the antibody? Hope someone can answer these questions for me, again sorry if its basic stuff! B