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Shadya

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Posts posted by Shadya

  1. We have used the Centron for two years and am really pleased. The company does an excellent job of hotline service and validation. It is easy to use. I like that the system is able to call on the phone and email alerts and that you can program for a number of phone lists/shifts/holiday schedules etc. We just got the web access installed a few weeks ago so we can even access programing and reports & graphs remotely now if we want. The variety of reports you can produce is impressive.

    Good luck with what you choose to do.

  2. We had the same rule: wait one hour post completion of transfusion if possible--I saw many examples of falsely elevated HCT etc. even though collected on the opposite arm with the tranfusion IV turned off. It really does take the blood a little bit of time to reach equalibrium. However, there are times (surgery, ER trauma, etc.) when this rule might have to be negated and just realize that the HCT etc. might be a little off.

  3. We are working on finding a way to document temperatures of fresh stem cell product during manipulation.

    Does anyone have some suggestions of some small temperature data loggers that would track the actual blood bag temp without breaking its integrity? What products have you found that work well.

    Thanks!

    I

    To my opinion, the best way of monitoring transport, is with a small electronic logger, that logs time and temp, and where the data can be filed with the rest of the monitoring data, so to create a waterproof

    traceability route.

    Correct, the loggers can be stored in a fridge, to achieve fast correct readings when placed in a cooler box, and prevent unnecessary out of range readings.

  4. We have recently began doing post-thaw testing and comparing it to the fresh testing, however, we are performing viable CD34 % recovery post testing. We are using a flow lab that tests for viable CD34 using 7-AAD and single platform methodology so I get comparable absolute CD34 values both fresh and post thaw. We have set a limit of 50% recovery, or we will alert physician of possible poor/sluggish engraftment outcomes. We also calculate the dose of viable CD34 cells/Kg after freezing as well, so the physician has that to look at too. We report the post freeze viability on our reports for information, but have no standard or cut-off limits set. Since this is a viability for all cells in the product (not necessarily CD34 cells), you could get very poor viability just because you have a high granulocyte percentage which died during the freeze but your CD34 cells are fine. That's why we chose to to use CD34 viable recovery.

    There are a lot of excellent articles that can be review on this subject on the internet. Dr. Rob Sutherland has put out quite a bit of research on this.

    Good luck!

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