We use solid phase to test 6 sample pools of our donors. Pos. pools are broken down and tested individually in solid. These samples are then sent to Ref. for Gel I.D.. We have several donors a week that are pos. in solid phase (many times husband/wife donating on same day) and neg. in Gel and tube(PEG). We are not automated for either system. We have a good deal of "false +" samples from solid and sometimes do not detect donors with 3 year history of antibody until posting. But this can be said about Gel now as well. As usual......everything has its + and =.
OPUS: how large are the donor sample pools you test? I'm currently doing some studies on different methodologies (2-cell vs. 3-cell, gel vs. solid phase) to determine which is the most sensitive for detecting Anti-D in pools of 512 and would like to know your experiences with pooled samples for antibody testing.
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Gel vs Solid Phase
in Transfusion Services
Posted
OPUS: how large are the donor sample pools you test? I'm currently doing some studies on different methodologies (2-cell vs. 3-cell, gel vs. solid phase) to determine which is the most sensitive for detecting Anti-D in pools of 512 and would like to know your experiences with pooled samples for antibody testing.