[DTT and DARA]

Thank you very much...

[DTT and DARA]

can anyone tell me the volumes I have to use to prepare a PBS with a pH of 8. I m following the instructions of AABB manual to prepare DTT and it requires PBS, pH= 8,0.    

THanks

[ABO Mismatched transfusion?]

Something is missing in this case. The patient received another AB transfusion 2 days later and crossmatch ok. One week later we prepared two units to the OR and one unit was incompatible and another was compatible. By that time he wasn´t transfused. The genotype of the patient is O1A1

[ABO Mismatched transfusion?]

A patient was admitted to our hospital with GI bleeding and Hb=4,4.

The forward blood group was determined with cards from Ortho Biovue system. And was positive with anti-A and anti-B. The reverse grouping wasn´t done. The crossmatch was performed in antiglobulin phase. 4 AB units compatible of packed red blood cells were sent. One week later the patient was admitted again and 2 units were requested. This time both units were incompatible and no unexpected antibodies were found. It was then repeated both forward and reverse grouping and a discrepancy was detected (forward: AB; reverse: A). Can anyone explain why the patient had no reaction to the blood transfused?

[Autoadsorptions with PEG in gel cards]

I need a way to do autoadsorptions in gel cards...

[Autoadsorptions with PEG in gel cards]

Does anyone has any experience in using the PEG autoadsorptions in gel cards? I need some guidance.

[Warm auto questions]

Anyone can help me out.... how to create a SOP TO study warm autoantibodies? What should I use? PEG? WARM?

[Elution problem]

Do you believe it´s necessary to do an eluition when your DAT is 12 and you have panagluttination? What can we miss if we only do adsorptions?

[Adsorption & Elution "Detail Lecture needed"]

Thanks for the info.

[Adsorption & Elution "Detail Lecture needed"]

Can you sent me too? love_blind@hotmail.com

Thanks

[Warm auto questions]

The truth is where I work technicians always complaint about the adsortion and eluition techniques. Spend hours and hours with this patients and don´t give straight answers about the outcome of this studies. We use gel in our routine and I believe that it was lost the habit of working in tube by the youngsters. They seem to have difficulties on choosing the right paths during those studies. wonder if you could give me some suggestions on literature to help me creating a SOP where the work out strategy could be established. The real doubt is do you use always the same strategy on this cases? Could you help me out. Tx

[Warm auto questions]

Malcolm, do you think we can use the genotype as our reference to transfuse the patient? For instance if you had the chance of a quick PCR would you rely on that information and transfuse the patient without doing adsorptions? Or would you use the information given by PCR to select cells for your adsortions?

[Weak D testing on donors]

In my hospital The Rh(D) type only in donors is determined with Anti-D reagent from two

different sources using a validated method.. If blood is typed as D-Negative

it should be tested to detect weak "D" using IAT method.

[Delayed transfusion reaction]

Hello. Can anyone share what is the policy in their laboratories regarding saving a sample from the donor unit to study possible delayed transfusion reaction? For how long do you keep the donor sample? Ty

[Routine cord blood testing (ABO, Rh, DAT)]

It´s hard tochange that policy... I was mother recently and though I informed the obstetrician that I and the father of the child were O Rh positive and my PAI was negative they still typed the cord and did a DAT. I believe that a huge group of the obstetric staff in my hospital doesn´t know the meaning of the tests...

[Hello from Portugal]

Hello from Oporto, Portugal