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Prilly

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Everything posted by Prilly

  1. As my lab is on the Fast Track to becoming a Joint Commission Lab, we are trying to get all our ducks in a row. The question on the table is 'What would be an appropriate way to perform Quality Control on Wright stain?' I would appreciate anyone sharing examples of how other labs take on this task.
  2. Gel is our primary, except for our repeat front types that we primary do in tube. Most testing is performed in gel and that is the choice of most techs. It would be rare to perform an Antibody Screen in tube, but not really a rare occurence to see a full type performed in tube. We do stock and QC tube reagents and they are available for use if need be. I just wasn't sure if it was a requirement to document, since I have documented at every other institution I have worked at.
  3. Can anyone tell me if it is a requirement to document what method the tech uses to perform the testing? (gel vs. tube) We utilize both methods in our laboratory. Currently we document the method in our log book (yes, we are still paper). We are in the process of installing computer software and validating it. I had in my head that it was a requirement, but wasn't positive. The software doesn't have a way to document this that I am aware of, so I am trying to see how creative I am going to have to be to stay compliant.
  4. Our semen analysis kit states: -If semen is viscous and difficult to pipette, sperm concentration will be difficult to measure accurately. Viscosity may be reduced by passing the speciment through a needle (18 gauge) several times with a syringe. Include a note on teh Semen Analysis Treport that the count may be inaccurate because the semen was highly viscous. (Source: Origio Humagen Pipets Package Insert for the Semen Anaylsis KitTM)
  5. Does anyone have a literature reference for the exact procedure of platelet estimations. Currently our procedure reads to count 10 fields (100x) average and multiply by 20,000. We recently got a new scope with a larger Field of View. Since the size of field has changed, shouldn't our multiplication factor change? At a previous hospital I worked at some scopes we multiplied by 20,000 and some 14,000 due to the change in Field of View. I am needing to update the procedure and wanted to make sure I had the correct value.
  6. We had a bad week similar to the one you are referring to. Make sure you call the ORTHO Hotline and file a complaint with your lot numbers. I don't have the info on the lot #'s I complained about but they keep record of all complaints, so they can monitor trends. The frustrating part is many times if you repeat the test it often will go away or appear as a false positive in a different well. After that week, I ALMOST switched everything back to tube ABO testing.
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