[Positive DAT on Cord blood intefering with weak D test]

What is a FTLS (not familiar with that acronym). Fetal Bleed screen? I would think a Fetal Bleed Screen would give you a false negative given that it is not labeled to be used as a screening test for weak D infants. Rather a Kleihauer-Betke should be done instead.

[Positive DAT on Cord blood intefering with weak D test]

Very interesting... thanks for that info.

[Positive DAT on Cord blood intefering with weak D test]

I agree. We perform K/B testing in the case of a "true" weak D positive cord blood because on the possibility of getting a falsely negative Fetal bleed screen and to determine dosage. But one might argue no Rhogam is necessary as the baby is probably Rh negative. I think the decision to give RHIG would vary among different OB/GYN's.

[Positive DAT on Cord blood intefering with weak D test]

How do other facilities manage RHIG administration when the test for Weak D on cord blood is likely falsely positive due to ABO incompatability induced positive DAT. In this case the RH status can not be reliably determined, but most likely is Rh negative as the reaction strength is equal to the DAT (w+).

[Whole Blood Transport from mobile drives]

We use manual methods (Sorvall RC3BP) and draw about 20-25 units a day. The standard says to cool continously towards 1-10 degrees (no plt production). Does this not apply if red cells are refrigerated within 8 hours anyway????

Thanks,

John

[Whole Blood Transport from mobile drives]

Ok great thanks

John

[Whole Blood Transport from mobile drives]

Our longest drives are typically 6 hours but for these we often ship blood back by courier about halfway through the drive. Then all blood is processed and stored in the refrigerator within 8 hours of the draw time.

[QC for Leukocyte Reduction]

Pall Medical does our WBC count testing for our QC. I can provide contact information if interested

[Whole Blood Transport from mobile drives]

Does anyone use or know of an acceptable substitute for wet ice when transporting whole blood in coolers from mobile drives to the component processing lab. The blood is centrifuged at room temp and is not for platelet production?

[Autologous Donation for Fusion Surgery]

I would appreciate comments on the following practice:

Automatic scheduling of patients to donate autologous units prior to spinal fusion surgery for the purpose of "stimulating" the bone marrow seamingly to enhance the grafting process. Would this be considered an acceptable protocol? Most of these patients are provided no information regarding their options for transfusion and most would not be adverse to allogeneic transfusion. Many would have preferred not to have had to come in (sometimes from quite a distance) to donate.

[Problems with Gel]

We have recently experienced several cases of seemingly strong positive reactions on gel that prove to be negative with tube testing. Has anyone else had similiar experiences?

[Chlorascrub Swabstick]

Does anyone use Chlorascrub swabstick. It is made by PDI and is 3.15%Chlorhexedine Gluconate and 70% Isopropyl Alcohol. If so, How did you validate it and how?

Thankyou,

John

[Donor Management for Syphyllis Testing]

How do you close a poll?

[Arm Prep]

Does anyone use Chlorascrub (PDI) for donor arm prep and if so how did you evaluate it relative to iodine procedures. Also the original FDA document regarding arm prep is no longer available because it is seemingly out of date, yet I find no replacement guidance. Does anyone have a copy of this they could email me?

Thanks

John

[Hbv nat, hcv nat, hiv nat]

Cliff,

I spoke with the FDA about reentry using HIV/HCV NAT and was told it would be finalized by the end of 2007. More recently I have read it is nearly ready to be finalized. The author of the draft clearly stated it was not for implementation as a draft document. We do not reenter donors but I do let them know of the pending opportunity to be reentered if applicable

John

[Hemocue Controls]

Our mobile collection staff runs all QC prior to departure 90% of the time

[Reporting infectious disease to Public Health]

We also had this question regarding reporting to Mass DPH. I've spoke with an epidemiologist there and was told that as long as a donor is counseled and referred to Medical follow up, the Physicians office should be reporting reportable diseases and this was sufficient. I have reported cases in which we were unable to establish contact with a donor.

[QC of coolers for transport of samples for infectious Disease]

Does anyone else routinely perform temperature monitoring of coolers used to temporarily hold and transport test samples collected on a bloodmobile?

[Deferral for military travel]

I was curious to know if when questioning donors who were in the Navy, do other people concern themselves with time aboard ship when counting exposure time? It would seem feasible that ships would resupply when in port (beef) and the potential exposure would be present when traveling at sea.

Thanks in advance for any insights,

John

[Hemocue Controls]

I would be interested to know what others who use the Hemocue for donor Hgb screening run for daily controls....ie how many levels? and what range they encompass? Thanks!

[Good Bye]

Best of Luck to you!!!!

[Taking Temperature]

We use the Kendall Genius 2 Infrared electronic thermometer which recently replaced an earlier model (Kendall 3000A). So far the older model appears to be much more reliable with regards to maintaing its calibration.

[Donor Management for Syphyllis Testing]

What do Blood Centers do regarding donor management when the donor is repeat reactive on Treponema pallidum specific antibody tests (such as PK-TP) but non- reactive on enzyme immunoassay (such as CAPTIA Syph-G) or other fluorescent treponemal antibody (FTA) confirmatory tests?"

[HPV Vaccine Deferral]

Standard 5.4.1A #12 Synthetic Vaccines:no deferral if donor is symptom free and afebrile

live attentuated vaccines have deferral periods of 2-4 weeks and unlicensed vaccines:12 mos unless otherwise indicated by medical director