Melanie Oliveira

Sounds like a dilutional effect. How big is the patient and how much plasma/platelets did the patient receive during the time frame between blood draws?
I have seen this with massively transfused patients and with plasma pheresis patients and with patients who have received multiple products over a few days.

Sounds like ABO discrepancy due to a cold agglutinin which may or may not have specificity.   I would run 3 M+ cells and 3 M neg cells at IS, RT and 4'C along with an autocontrol.  IF only the M+ cells react at IS or RT, then I would ficin treat the A1 and B cells and repeat the reverse grouping using ficin treated cells.   Just a suggestion.  

Sounds like ABO discrepancy due to a cold agglutinin which may or may not have specificity.   I would run 3 M+ cells and 3 M neg cells at IS, RT and 4'C along with an autocontrol.  IF only the M+ cells react at IS or RT, then I would ficin treat the A1 and B cells and repeat the reverse grouping using ficin treated cells.   Just a suggestion.  

You can't use REST adsorbed plasma to XM.  IT removes ABO iso hemoglutinins.  But use your validated computer system if all else fails.   If your computer is validated and will not let you select ABO incompatible units then it can be used for ABO compatibility.