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BBNerd

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    BBNerd reacted to Malcolm Needs in Prewarm ABO   
    Well, for the simple reason that 1) you have to prove that the reverse grouping anomaly is really just due to the "cold" auto-antibody, and not to something else, and 2) that there is no clinically significant atypical alloantibody being masked by a "cold" auto-antibody of wide thermal amplitude.
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    BBNerd reacted to Malcolm Needs in Prewarm ABO   
    What we do is wash the red cells of the patient's sample multiple times with warm saline.  Then we would use the ABO antisera at 37oC with positive and negative controls at the same temperature.
    The problem comes with the reverse grouping.  If the "cold" auto-antibody has a wide thermal amplitude, it may be necessary to use cord cells at 37oC, if you can get hold of them.  In the UK, this is no longer easy, as we have to get written consent from at least one of the parents.
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