Awambold
Content Type
Store
Profiles
Forums
Blogs
Events
Frequently Asked Questions
Gallery
Downloads
Glossary
Links Directory
Questions
Jobs
Vendors
Posts posted by Awambold
-
-
I was a key operator at my last job for the erytra. Fantastic instrument! It is incredibly quick, easy to understand, and I had very few errors with it's use. Also, TRUE stat function! We did have a problem with HLA antigens in our screen cells, that lead to a lot of unnecessary panels being run. After contacting Grifols, they quickly adjusted their donor screening process and the subsequent lots were clean. I was very satisfied with their customer service, and willingness to modify their products and software for their clients.
Eluate
in Transfusion Services
Posted
Are you using the Gamma ELU-Kit ?
#9 in the Limitations talks about the possibility of false positives with strong, high titer antibodies. The saline wash at the start should remove any allo antibodies in the plasma, but some nonspecific binding can allow some antibodies to linger. The LISS in the wash solution will hold them onto the cells during the rest of the washing steps and they will show up in the eluate with a clear last wash. If your plasma reaction was 4+ and your eluate reaction was 2+ or 1+ then its more likely a diluted allo antibody than an auto anti- C. I've seen this happen twice with C.