Michelle R

Interesting update. Received a very small amount of additional sample and performed a manual GEL (BioRad) crossmatch and it was compatible. Decided to try a 3% 3 cell screen (Immucor) converted to 1% for GEL testing and it was also negative. Very different results since we had been seeing pan reactivity in manual GEL previously (about a week ago). Possible preservative antibody that is neutralized (?) or non reactive in LISS tube? Hoping for more sample to perform repeat testing, maybe whatever it was has gone below detectable limits?

Interesting update. Received a very small amount of additional sample and performed a manual GEL (BioRad) crossmatch and it was compatible. Decided to try a 3% 3 cell screen (Immucor) converted to 1% for GEL testing and it was also negative. Very different results since we had been seeing pan reactivity in manual GEL previously (about a week ago). Possible preservative antibody that is neutralized (?) or non reactive in LISS tube? Hoping for more sample to perform repeat testing, maybe whatever it was has gone below detectable limits?

We were thinking an anti-I initially as well but we didn't find much literature about a premature baby forming an anti-I at birth since it appears to be IgM. We were also wondering about other possible antibodies that would be non-reactive or weaker with cord cells. We requested Immunoglobulin tests and received the results today so I will post them for additional information. IgM is double the upper value for the range??
IgM= 45 mg/dL (range for <1 month old is 4-20 mg/dL); they repeated to confirm IgM= 47 mg/dL
IgG= 519 mg/dL (range for <1 month old is 649-1627 mg/dL)
IgA= <6 mg/dL (range for <1 month old is 0-0 mg/dL)