[solid phase associated antibodies/ Jka antibodies]

My institution has been using Capture for close to 10 years. We have been using the Galileo for 3 years. We've been satisfied with the Capture system and the Galileo. I think with any new procedure, there's a learning curve...especially when the new procedure requires subjective interpretation. Performing antibody IDs on an instrument such as the Galileo might improve the consistency of results. We perform a Capture panel when the Capture screen is positive either manually or on the Galileo. We have observed an increased incidence of anti-Jka. Some of these cases were not reproducible using tube testing(we use PeG). None of the patients were OB patients. Since Capture is sensitive, we expect that this may happen. We honor any antibodies ID'd in Capture even if negative with tube testing. We also agree that because of the sensitivity of Capture, it generally cannot be used if the patient has an autoantibody. As far as the patient having a positive JKa typing, I agree that we would need more info (recent TX, DAT results).

[ABO discrepancy with CORD specimen]

The "A" antigen in newborns is not usually well developed. This fact occasionally results in a weak A typing. You can't test for a weak A subgroup in newborns, the test results are not valid. You might not be able to resolve this problem until the baby is older.

[Anti-A and Anti-B titer in O group platelet]

We perform an anti-A and anti-B titer using a 1:100 dilution on all O apheresis platelets. We incubate at RT for 15 minutes. If the result is negative, we use the unit for non-O adult patients.

[exp. of pooled 2-bag pheresis plt]

Does anyone have a policy regarding expiration time on a Platelet Pheresis product once a 2-bag collection has been pooled into one bag?

If a platelet apheresis product is divided into 2 bags, once the product is pooled into one bag, the expiration is 24 hours. We pool into one bag and change the expiration at issue.