Posted July 6, 201213 yr comment_45112 We have recently moved our testing to new Ortho analyzers. To get the result reported out as "Neonatal Bilirubin", the new analyzer measures total bili (82247) and direct bili (82248). Then a calculation is made with those results, conjugated and unconjugated bili and a delta bili to produce the neonatal bili result. We were told to report the end result using code 82247. Does anyone know if that is correct?
July 7, 201213 yr comment_45130 This is the way Ortho (Kodak) has advocated for 30+ years. There method does include the Delta Bili that liquid reagents do not include. The method works very well except when you transfer a neonate to regional NICU whose lab uses liquid reagent methods--the coorelation of neonates is not that good. Overall though, my experience with this method vs liquid is much better. The test CV for the slide method is excellent.
July 9, 201213 yr Author comment_45143 Thank you...sounds like a good move for us. Can you please affirm that you're reporting only the 82247 CPT code for the result, however?
September 15, 20177 yr comment_70875 The Coding and Payment Guide for the Physical Therapist (CPT) is a numeric code that codify for specific analyte determined by means a specified methodology with a automated or manual procedure that for Bilirubin Total & Direct is 82247 for Bilirubin total (Neonatal Bilirubin) and 82248 for Bilirubin direct when measured with Jendrassik-Grof modified methodology applied on automated instrument. Through Bilirubin total reagent are measured in single determination conjugated, unconjugated and delta bilirubin (neonatal samples contain little or no direct δ-bilirubin) that produce a single result Bilirubin total (Neonatal Bilirubin) identified with 82247 code in CPT, therefore your approach is correct. I for measure neonatal bilirubin employ plasma obtained by capillary whole blood and as method a Jendrassik-Grof modified methodology two liquid stable reagents (Sulfanilic Acid Reagent : dissolve 5.58 g [32.2 mM] of sulfanilic acid to 15.5 -16 ml of HCl conc. 37% fuming and dilute to 500 ml con dH2O. Mix 250 ml [3.5 M] of DMSO with 250 ml [4.44 M] of ethylene glycol and fill up to 500 with dH2O. Mix the DMSO/ethylene glycol solution with the sulfanilic acid solution and store in polyethylene opaque at 4 °C stable 1 year. The solvent mixture of dimethyl sulfoxide, and ethylene glycol besides as solvent for the total bilirubin assay, eliminates interference from hemolysis [neonatal plasma] up to 10 g/L of hemoglobin; Sodium nitrite solution pH 8.0 : dissolve in 60 ml dH2O, 0.752 g [109 mM] of sodium nitrite, and 0.240 g [16.9 mM] of disodium hydrogen phosphate anhydrous [Na2HPO4], then 0.010 – 0.040 g [0.96 mM] ethylenediaminetetraacetic acid tetrasodium salt dihydrate, and fill up to 100 ml with dH2O with final pH about 8.0. Store under refrigeration, at 4 °C, in brown glass-stoppered bottle, resulting stable for 1 year, but must be discard if it becomes tinged with yellow. In aqueous solution the sodium nitrite is immediately degrade and convert itself to nitric oxide, while in a pH neutral or slightly alkaline solution, the sodium nitrite is stable). Ratio reagents SAR:sample:SNS is 1:0.1:0.01 and read Abs at 555 nm
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