QC testing using Nageotte chamber

[SLBCNW01]

Can anyone share what they perform for daily QC when counting residual WBCs using the Nageotte chamber?

[RR1]

I give up...what is a Nageotte chamber .....is this just a counting chamber?

[Marilyn Plett]

The Nageotte chamber is designed for counting very small numbers. I would suggest sending your samples to a laboratory that uses flow cytometry to count residual WBCs. Counting in a Nageotte is not worth the effort.

[clmergen]

QCing a Nageotte count would be like QCing a manual diff. It isn't necessary as far as I know.

[mclin]

I found an article referring to your question, hope it helpful.

http://www.cbbsweb.org/enf/2003/qcnageotte.html

[dmorrissbb]

We use a product by R&D Systems (www.rndheme.com) called Leukoreduced Combined Controls.

R&D LeukoReduced RBC/PLT Control

LeukoReduced RBC/PLT control is a bi-level control product designed to monitor flow cytometer and Nageotte Chamber methods for quantification of residual leukocytes in LeukoReduced red blood cell and platelet products.

Features:

• 75-day closed vial stability; 2 QC months
  
• 30-day or 21 thermal cycles open vial stability
  
• Packaged in 3mL pierceable ***** cap tubes
  
• Assayed for Flow Cytometer and Manual Methods
  

[hind]

1- to use nageotte chamber the sample should be stained with turk's solution to get best results.

2- after staining, pippetting 100 microlitter into the chamber and leave it for 15 mints.

3- under the light microscope count just the first 5 rows..

4- use this equation to calculate the residual WBC's:

(WBC's count / 5) x 1000 x blood unit volume ml

********

WBC's count = from the chamber

5 = # of counted rows

1000 = volume of mixture(100micro of sample+900 micro of turk's solution)

blood unit volume= (weight of the blood unit - weight of empty blood bag) / 1.1 (specific gravity of RBC)

Edited February 1, 2010 by hind

[OPUS104]

We are a small blood center (about 85K draw a year) and were just "dinged" by AABB for not performing QC on our manual nageotte chamber testing. So I guess THEY think it is necessary. We have to have most of our counts within a couple of hours for product release, so send-outs are not an option. We do not do enough of them daily to justify a flow either. I guess we will start with dmorissbb's reagent and see from there.