dalangsfor Posted November 17, 2005 Share Posted November 17, 2005 We frequently process blood collected from the day before. Our Transfusion Service frequently says that these units have too much plasma still on the units. We obviously don't make platelets from units that have been refrigerated overnight. Has anyone ever calibrated a centrifuge for the purpose of processing cold units and what information are you trying to capture? I am not interested in platelet yields since we do not make platelets from these units. I just need the speed and time to be such that we are expressing the most plamsa possible.Thanks Link to comment Share on other sites More sharing options...
Lcsmrz Posted November 18, 2005 Share Posted November 18, 2005 Your centrifuge manual should have an RCF chart or a recommended starting point for hard and soft spins. For hard spins, I would leave the RPM setting fixed at your current setting and tweak the time settings up and down a bit and see what happens to the cold unit's hematocrits.The quickest time reaching the best hematocrit is your optimum setting. It could be different for each centrifuge ... Link to comment Share on other sites More sharing options...
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