Vision DAT testing

[DonnaT]

For those that perform Poly DATs on the Ortho Vision- What do you use for controls?

We currently perform poly DATs manually in gel. We run an IgG positive control, a Complement positive control and a negative control for poly DAT cards. 

 

Thank you, 

Donna

 

 

[NicolePCanada]

We do the same, but we run them on vision. The IgG pos is 300uL of coombs control cells and 900ul of MTS diluent 2. The C3D is the same with complement control cells. For our negative control we use on the Panel A cells that aren't included in our mini panel. We use Bio Rad Diff Dat cards if the Poly is Positive. Those we pipette manually on the bench. Just waiting for Ortho to get a differential DAT gel card that runs on the vision. Then it will be great.

 

[Townsend]

We run our Poly and IgG DATs on the Vision.  We make up our own in-house pos/neg DAT samples as recommended by the Ortho rep.  Here is our procedure to prepare those tubes and labels below.  If you don't have an SCD, you might be able to enter a unit that was expiring, but you'd have to establish some sort of expiration for the cells/sample then:

1.   Select an Rh Positive red cell unit with a good outdate from the available inventory.

2.   Complete a label with the lot “DPMMYY” (using MMYY as the month and year of preparation; i.e. “DP0521”).  Add the expiration date equal to the expiration date of the red cell unit as well as the date prepared and your initials.  Place a barcoded P10000 LIS accession label on the vial so it can be scanned onto the Vision.

3.   Complete a label with the lot “DNMMYY” (using MMYY as the month and year of preparation; i.e. “DN0521”).  Add the expiration date equal to the expiration date of the red cell unit as well as the date prepared and your initials.  Place a barcoded N20000 LIS accession label on the vial so it can be scanned onto the Vision.

4.   Connect a syringe set to the red cell using the Sterile Connection Device and remove approx. 10mL of packed cells.  Place the red cells evenly into two plastic 12x75mm test tubes (one labeled P10000 and the other with the N20000 label made above).

5.   Add 2 drops of Anti-D (Ortho Bioclone) to the P10000 packed cells, mix well, and incubate for 30 minutes at 37°C.  Mix again about half-way through incubation.

6.   Test each of the prepared red cells by performing a Poly DAT in gel on the bench.  Ensure that the results are 1-3+ on the P10000 cells and negative on the N20000 cells prior to placing the vial into use.