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HemosIL D-Dimer HS 500 on ACL top

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Hello everyone, I have a question as a med lab Hematology student. 

Does anyone have experience using the HemosIL D-Dimer HS 500 latex enhanced immunoassay on the ACL top machine, where the manual pippeting of 1000ul patient sample is required?!

If so would you know what effect expelling the patient sample to the first stop on the pipette, therefore leaving a tiny amount of sample in the discarded pipette tip would have on the result?! Thank you!

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