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High Performance Liquid Chromatography (HPLC)

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Hello every one, 

In hemoglobin chromatography (HPLC), we sometimes get small, insignificant peaks. Like Hb-S peak of 8.0, Hb-C peak of 7.5 (RT 180 secs). The only things which we do are to either retest with same sample or ask for fresh sample. Point is, the hemograms and histories of such cases have not been helpful up till now. Is there anything else which could be done?



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  • 10 months later...

U need to exclude recent transfusion or carry over from previous sample ( although the levels mentioned is high for carry over)

wise if above mentioned are excluded to consider genetic testing and parental  screening 

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    • By mminhas44
      Hope you are fine. My problem is:
      Which other hemoglobins elute in same window (same retention times) as Hemoglobin S in ARKRAY ADAMS A1c HA-8180T Analyzer?
      (P.S. it gives retention times in seconds.)

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