Sysmex ColorWright Stain used manually

[Kelli C 0]

Hello,

 

Has anyone use the Sysmex ColorWright Stain for manual differential slide preparation off line.    Currently we use the Diff-Quik system for our manual slide preparation and wondering if we can use the stain from the SP10? 

[kimannez 6]

We do that when troubleshooting stain and SP issues to help isolate the causes of issues.   Basically use Coplin or other stain jars with undiluted Stain then another with 1:10 diluted stain/buffer then a water rinse.   I’d start with the same stain times as you have on your SP then try it out with some normal and abnormal samples and adjust manual timing as needed to get desired staining.  You would also need to decide how long you use the stain and stain/diluent mix before replacement.  Since we are doing this for troubleshooting typically we dump it shortly after we're done, but you may be able to 1-2 days if you're keeping it covered.