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  1. Hi All Female patient not childbearing age with historical A POS no antibodies detected subsequently multi transfused. After transfusion of 2 x O NEG units a week later a sample tested no antibodies detected by IAT however cross-match request was performed by IAT and both units were positive Further testing DAT +1 and panel cells enzyme ONLY reactive +2 with R1R1 cells and the rest of the panel cell reactions +4 ABO reverse grouping showed A cells +2 (conclusion therefore possible A subtype with a no specific antibody or reaction with cde in reverse grouping reagents due to an anti-c) Reference Lab Results Non specific anti-E and anti-c detected in enzyme IAT, DAT IgG 1+ As multi transfusion advise was cross-matching group O E-c-K- red cells Genotyping results to follow. Would anyone agree with the reactions with the reverse grouping agents and if they have seen this was the anti-c detected been by IAT/and or Enzyme Thanks

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