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Hello Blood Bankers, I recently ran into my first unsatisfactory Antigen typing QC. A tech performed QC for an "e" antisera using a heterozygous cell. Results were negative at IS and negative after 5 min incubation. This was repeated by the same tech and later repeated by myself:Results did not change. The "e" antisera was ran with a different heterozygous cell and results were negative at IS and 2+ after 5 min incubation. Both the cells and the antisera were near expiration date. However, I'm a bit confused as to what corrective action is needed. How do I prove my panel cells are working correctly and how do I prove my antisera is working correctly? I think I proved my antisera is working correctly by running it with another Hetero cell. Should I run my panel cell with a new antisera to prove the panel is "e" positive? Thank you, Serafin
