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Showing results for tags 'biorad'.
Hi All, I am about to venture into performing my first ever validation of a test. I have been tasked to validate Rh+K phenotype testing on the BioRad IH-1000 (two of them). We have been, until now, performing the test manually, but as work is getting busier, performing it on the analyser might prove easier (or at least motivate people to perform phenotype). I have been given advise by my senior on how to go about it: Select 10 Donor Red Cell units which have Rh+K phenotype performed Perform the phenotype manually Perform the phenotype on the analyser compare the result pat myself on the back, provided I don't mess it up The issue I have is donor red cells doesn't indicate if they are K+, and I wanted some K+ as well as K-. I could always keep testing a lot of donor units until I come across a K+ unit, but I don't want to was a lot of cards (but that might be my last option). If I choose the NBS or BioRad Antibody Panel Cells, then the issue is the strength of the test cells, as they are, I think, 0.8%, and it does not fully represent the way we perform our phenotype manually, as it uses around 5%. I can always try and make the strength of the solution stronger, that is another option. So this is where I am at. If anyone has a suggestion, or better a complete plan, then I am happy to hear it. Also if there is any point I missed or need clarification, please feel free to ask, I'm fixed to this specific thread all night long. Cheers, Jermin
Dear All Can I pick your brain about something. Under the UK Guidelines, there is no requirement to perform a post-partum antibody screen on the mother, and no requirement to perform a DAT on the baby if the mother had no antibodies during her pregnancy (or in the past), and the mother was given routine anti-D immunoglobulin prophylaxis during her pregnancy. We are introducing RAADP in The National Maternity Hospital this year and we are wondering how did labs in the UK approach dropping the DAT on the cord. We use BioRAD 6 well ID-Cards (DiaClon ABO/D + DAT): A, B, DVI-, DVI-, ctl, DAT As we sometimes (not often) see one D well negative and the other D well positive we would prefer to stick to the 2 D well ID-card. Our problem is that we cannot source a BioRAD ID-card that has this minus the DAT well. What do BioRAD users in the UK do? Any advice would be greatly appreciated. Best wishes John