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Found 3 results

  1. Hi all, I'm still a relatively new blood banker and was wondering if you all would be willing to shed some light on the different methods of blood banking? We currently use solid phase but will be switching to gel next year. I have some questions regarding each method but also wanted to see what seasoned techs have experienced with them. Solid-phase: Incubation @ 37 with potentiator Wash AHG Read Pros: Sensitive Cons: Question: Won't pick up on clinically insignificant cold autos because of less exposure to cold temperature during the process? Or is it due to something else? Good to use with insignificant cold auto if you don't want to pick them up on screen? ______ Gel: Incubation @ 37 on top chamber, with potentiator? No washing Spinning down through the matrix containing AHG Pros: Very sensitive Cons: Will pick up insignificant colds due to the chamber temperature and matrix can catch IgM? Rouleaux Notes: IgM remains as there's no washing _______ LISS Tube (skipping IS phase): Incubation at 37 with LISS Reading (for strong IgM or IgG) Wash (removing unbound Abs) Add AHG Read (for IgG) Pros: Not too sensitive, not too weak Cons: Won't pick up weak antibodies Question: Won't pick up insignificant warm because LISS isn't strong enough? (Such as weak warm auto) Good to use when not wanting to pick up warm auto on screen? ________ PEG Tube: Read at IS (for IgM) Add PEG, incubate at 37 (30min inc. might pick up insignificant Abs?) Wash Add AHG Read (for IgG) Pros: Strong than LISS Cons: Please feel free to share any thoughts or experience.
  2. Hi I am reference coordinator at 450 bed Level 1 Trauma Center. The last few months I have seen a few times when all three Neo screening cells are about 2-4+ pos and then Neo panel was all negative. These patients did not have Rhogam and tested negative in PEG testing. I have also seen this happen with several different Neo screening cells lots. One patient screen on Lot R369 was 3+ pos for all three cell on both Neo instrument but had a negative Neo panel on Lot ID201. Then this patient was tested against another screening Lot (R374). Which resulted in 3+ reactions on all three cell on one instrument but the screen was negative on the other instrument. 30 min Peg screen and auto control was negative. Immucor is reviewing this one but has not responded yet. Another patient example is a patient screen on Lot R384 tested 3+ on cell 1, 2+ on cell 2, and ? on cell 3. Since the other Neo was down the Tech repeated the screen with Manual Solid phase and all 3 screening cells were 3+ pos. The Neo Panel DN072 was all negative and so was PEG screen and panel. Has any one else had a solid phase panagglutinin positive screen and corresponding neg solid phase panel?
  3. Hi Everyone, I am new to this site, but could not find any threads specifically pertaining to some of the peculiar solid phase issues my lab seems to deal with. We have one Echo, one Galileo, and a solid phase station. We have been using them for a number of years. Like many people have posted on the forum, there seems to be some strong panagglutiniation “false positives” but ours only appear on the machines. Interestingly, if we run these samples in manual solid phase (MSP), both the screens and panel cells will be negative (or show an underlying antibody). Warm autos are then ruled out. If it is still a panagglutinin in MSP, we move to gel and follow through with warm auto testing. Though MSP is supposed to be a little less sensitive than the machines, a 3+ does would not normally turn negative. I am posting to see if anyone has the same experience, where machine solid phase testing is all positive and manual solid phase testing is negative. This leads me to believe that the “solid phase antibody is actually an antibody to the pre-embedded screen and panel strips because there is no other difference between the two testing methods. Does any one else go to manual solid phase before going to their secondary method?
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