[N Lectin/Vicea Graminea seed availability]

Why don't you just test treated and untreated cells in parallel with a normal anti-M or anti-N?  The untreated cells will be positive and the trypsin treated cells will be negative.  Won't they??

Thanks for your response!  I agree that this seems like a logical "check" of treatment and we tried this with Anti-M and the cells were still weakly positive.  I don’t believe that the antigens are always destroyed, but are “altered.”  We did not feel like this was a good test of treatment as it could be very subjective and not as clear cut as N negative becomes macroscopically positive.  While the literature I read does state that the M and N antigens are denatured, this was not a suggested method of checking the enzyme treatment.  The published method is to test treated N negative cells with N lectin.  Since the publication tell us to use N lectin, we were hoping to find another source of N lectin so that we would not have to complete a huge validation on another method that is not as reliable. 

[N Lectin/Vicea Graminea seed availability]

I purchase anti-N lectin from HemoBioscience

We currently purchase from them as well.  We were informed that they are no longer going to be producing/selling N lectin.

[N Lectin/Vicea Graminea seed availability]

Trypsin is a valuable enzyme used in our IRL.  In order to determine if treatment is successful, we test trypsin treated reagent red cells with N lectin (Vicea Graminea).  Trypsin treated N negative red cells test positive with N lectin.  Unfortunately, we have been informed that our current suplier of N lectin is stopping production.  We are in search of a new source of N lectin and are wondering what other labs are using.  We have procedures for making the lectin ourselves, but we are unable to find a supplier of Vicea Graminea seeds.  Any information is appreciated.