We had a patient come in that had a history at our hospital of an anti-Bga. This was from AHG tube testing using LISS. We now use capture technology on an Echo and the patient came in again. Screening cell two on the Echo was positive. Our SOP is to then do a ReadyID panel and a DAT also on the Echo. The DAT was negative and all E+ cells showed reactivity on the panel. (Screening cell 2 was also E+.) We then did two AHG phase crossmatches on the Echo which were both compatible. The problem came when we phenotyped the patient. They typed as E+…as did both units that were crossmatched. Our manager ended up calling it an autoantibody with anti-E specificity. I am fairly new to blood banking but wondered why she called in an autoantibody if the patients DAT was negative. I believe I read something that a person can create a non-red cell stimulated anti-E but could cross react with red cell-bourne structures. I was just wondering what everyone out there thinks about this or if anyone could just shed some light on this for me. Thanks!
