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Posts posted by fmatheu
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What formula did you use when you used HCT. I am going to start leukoreducing and I want to do it with HCT first and compare it to weigh.
Percent Recovery
in Donor Services and Donor Recruitment
Posted
Here is the formula we use for percent recovery for frozen red cells. I could use the same formula, but I do not want to use HCT for LR. I want to know where the .0693 comes from in my density formula. It was established by the Freezing Protocol, FDA does not have a problem with it. I am training someone and he asked where the number comes from. I can't explain it. Do you know?
Pre-GlycerolizationDeglycerolizationWeight266371Hematocrit7549Density1.0569751.038957RBC Concentration251.66357.09RBC Volume188.75174.97Percent Recovery92.70% Use HCT in decimal format. Use 75% for the pre-glycerolization HCT.Density (calculation = (0.0693 * HCT)+1.005)RBC Concentration = (Net Weight/Density)RBC Volume = (RBC Concentration * HCT)Percent Recovery = (Deglycerolization Volume/Preglycerolization Volume)