sandoq

Hi guys, Thanks for all the responses. Patient is type: B Rh+ I tested the cells with anti-H : + We found out that the patient had a history of multiple abortions!!!!! which lead us to suspect anti-PP1Pk (p), fortunately our lab had a few cells that were PP1Pk - and they tested negative for the patient. Conclusion= anti-PP1Pk.

Hi everyone, I need your help in figuring this one out:cries:. patient is Hispanic; Autocontrol: negative DAT= negative All screen cells(3 cell panel) and antibody panel positive: all tests ran in PeG at 37C immediate spin= 2+ coomb's phase= 3-4+ all cells; DTT treated cells= same reactions(resistant) Enzyme(Papain)= enhanced reactions(resistant) I tested with Lub-, I-, Vel-, Jk(a-b-), P- cells but all same reactions= 2+ immediate spin (RT), and 3+ AHG phase; Also tried testing patients cells with anti-Ge2, -Ge3, -Dib, -Wrb antisera =all tested positive for the antigen. Any suggestions?? Let me know what to do next? Thanks

Thank you so much for the response. I am still looking a reference lab (preferably in the US) that can do ABO DNA testing??

Can anyone help me out with this one: 17 year old healthy male donated blood. No history. The ABO typing is as follows: tube method (forward) ortho anti-A= 4+ ortho anti-B= 2+ (reverse) A1 cells= negative A2 cells= negative B cells = negative (immediate spin), 2+ after 15 min room temperature incubation. we tested with anti-A1 lectin= negative; acidified the anti-B to a pH=6.0 still gave 1+ reaction. I was leaning towards "acquired-B antigen", but I think it maybe something else because the donor cells still reacted with the acidified anti-B?? Can anyone help me out? any suggestions would be appreciated..Any reference labs that do ABO molecular out there? thanks sandoq