[transfusion of lipemic blood ????]

i **** to know about the lipemic blood!!! it should be accepted for transfusion or should be discarded??

and what ever the answer i want to know why??

& thanks

[QC testing using Nageotte chamber]

1- to use nageotte chamber the sample should be stained with turk's solution to get best results.

2- after staining, pippetting 100 microlitter into the chamber and leave it for 15 mints.

3- under the light microscope count just the first 5 rows..

4- use this equation to calculate the residual WBC's:

(WBC's count / 5) x 1000 x blood unit volume ml

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WBC's count = from the chamber

5 = # of counted rows

1000 = volume of mixture(100micro of sample+900 micro of turk's solution)

blood unit volume= (weight of the blood unit - weight of empty blood bag) / 1.1 (specific gravity of RBC)

[total protein of washed LR-RBC's???]

plz.. for the QC on washed LR-RBC'S

i need to know how to calculate the total protein after washing LR-RBC's ?? i need the equation.

and the amount of total protein post-washed ?