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May 2024 Challenge

Cornelia Kuehn

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Posts posted by Cornelia Kuehn

    QC on blood group analyser

    in Education / Quality

    Posted

    The guidelines are the same as for tube testing. Each reagent must be tested on each day of use according to the manufacturers recommendations or meeting CAP/AABB standards. As for instrument specific QC, follow the manufacturers recommendations like you would any other piece of equipment.

    thank you very much for your reply

    Cornelia

    QC on blood group analyser

    in Education / Quality

    Posted

    Hi Cornelia,

    Forget about any regulations, and go back to doing what would be the correct thing regarding controls. Each of your antisera and reagent cells must be able to demonstrate positive and negative reactivity. Together they must be able to allow you to interpret the result to a conclusive blood group.

    With antibody screens, you need to use weak controls that demonstrate that any fragile antigens can be detected (anti-Fya) throughout the shelf life of your reagent. Additionally, each of these screening cells should be tested to show pos/ neg reactions.

    If your equipment uses a wash phase, you should be able to prove the efficacy of this procedure- by demonstrating there is no residual plasma proteins that could cause false negative reactions- during each wash cycle.

    We all need to 'go back to basics' with handling of controls. Simplify and do the most logical thing- then you can't go wrong.

    Rashmi

    I absolutely agree with you back to basics are the best. And if we do not have any FDA regulation your answer will fit perfect- thank you for that comment .

    type and screen

    in All other topics

    Posted

    Davif

    thanks for your reply. Here in Germany we do the procedure as followed. ABO+ Screen from a patient. If the screen is negative and the patient needs a transfusion we make a x-match ( not electronic ) The x-matched donations are 72 hours valid. if the patient hasn`t get the x-matched donations within the 72 hours you have to match them again. But we use every time a new sample from the patient.

    Cornelia

    type and screen

    in All other topics

    Posted

    hi

    just a question from my side - we do not perform the type and screen test here in Germany so I am not shure as I am right informed . Type and screen means you perform from a patient a ABO and an antibodysreen at the same time . If this patient will get 3 times a week a donation you`ll repeat this procedere 3 times- do I understand this correctly ??

    thanks for your answers

    blood-group.com

    in Education / Quality

    Posted

    blood-group.com is a new communication platform for the exchange of knowledge in transfusion medicine in Europe

    Feel free to visit the homepage we are online since 2 weeks- we also need your input and the exchange of experience from the US - it would be helpful to link the websites...

    Automation

    in Equipment

    Posted

    Hi Cornelia,

    I don't know much about the Tango - does this use liquid screening cells that are adhered to microplates or is the microplate pre-coated as for the capture screen?

    Thanks

    Rashmi

    Hi Rashmi

    the TANGO use the microtiterplate technology with liquid screening cells not precoated as the capture method the specificity is better and here in Germany were I am from we have some studys if you are interested in I`ll send it to you

    regards

    Cornelia;)

    ECHO- just a question

    in Equipment

    Posted

    hi, evryone

    just one question concerning emergency samples running on the Echo . If the machine is in a workprocess and you`ll run an emergency sample how long ( in worst case ) does it take until you`ll get the result of this sample? And what happend with the tests from the other patient did they get lost - in case of the interruption through the emergency sample

    thank you for the answers:whisper:

    Please help me to get compatible blood ?

    in Transfusion Services

    Posted

    I am lab technician and work in a hospial based blood bank in Vietnam. I have some difficulties and need your help. A patient need transfusion but we can not choose compatible blood for her although we have done X/M with all the blood bags in the stock ( about 50 bags) but all are incompatible with the patient's plasma AHG(+) . Patient with DAT(+), IAt (+), AC (++).

    1.Please tell me how can I get compatible blood for the patient in this case?

    2. In Vietnam, We do not perform Antibody identification for the reason of high cost , Do you have any solutions to solve this case?

    3. We are advised to transfuse to the the patient with the small amount of blood. Is that right and acceptable?

    Thank you very much.

    The transfusion of incompatible blood bags is not really acceptable you can transfuse blood with a positive Antibodyscreen from the patient without identification only if the results of the X/M are negative!!

    Blood Bank Automation

    in Equipment

    Posted

    I feel it is very important to consider what you are looking to accomplish in your lab and consider which instrument is the best fit for you based on your unique needs. I believe there will be an article posted in a trade journal by Suzanne Butch from the University of Michigan this October comparing blood bank automation -the Tango, ProVue, Galileo and Echo. There are only 4 blood bank automated platforms that I am aware of that are available today...researching all 4 to determine the best fit is the right thing to do.

    I absolutely agree with you the system has to fit in your lab and your workflow.... by the way you talk about an article can you give me an idea were I can get this

    regards

    Gel card with 1+ reactions showing negative after spinning a second time

    in Immunohematology Reference Laboratories

    Posted

    An ER trauma patient's plasma showed 1+ reactivity in Gel with Screening cell 1 of Immucor's Trio screen. The other two cells were negative. (We buy 3% Immucor screening cells, then dilute them to 0.8% for gel testing.) Using an Ortho 0.8% panel for antibody identification, we found 7 of the 11 cells reacting 1+ with a negative auto control. The reactions did not fit any pattern what-so-ever. Our workup was stopped at that point because the patient was transferred to a larger facility. On follow-up the next day, we found that the other facility had gotten a negative antibody screen, also using Gel cards. Within three hours of performing the antibody screen on the ER trauma patient, the remaining three wells of the gel card were used to perform an antibody screen on another patient. So, the card with the positive Screening cell 1 was spun for another 10 minutes. After the 2nd spin, the reaction had changed to negative. Later, we spun the two cards containing the antibody id with the seven 1+ positive reactions. After the 2nd spin (for a total of 20 minutes) most of the reactions were now negative with the others only questionable, definately not 1+ as after the orginal spin.

    I've checked the rpms and timer on the centrifuge, and they are okay. Has anyone else seen positive reactions change to negative after spinning a second time?

    the problem is if you spin a second time you change the method by itself and you get a result that you cannot validate..... but I think you have to look if the patient have autoantibodys it seems like you have to eluate and see what is on the surface of the ery`s

    Automation

    in Equipment

    Posted

    For those of you who have evaluated and subsequently gone with automation, what criteria swayed you to one instrument or the other? Any problems encountered? All insight would be greatly appreciated! We are currently looking at the Provue and Echo...we are gel system users right now and very comfortable with it.

    Laurie,

    I think your decision depends a little bit on the throughput of the machines. An Echo and also the Provue are equipment for smaller labs - and if you think about Echo you are thinking about an other technology so I think you have not really a problem to switch. So the Tango is mayby also a possible opportunity because the solid screen method running on this system for the Antibodyscreening has a higher specificity than the Capture technology .

    I am from Germany and we have made here a direct comparison between Galileo and Tango and that was one of the results of this benchmark

    Diamed & Medion Diagnostics

    in Transfusion Services

    Posted

    Has anyone had any experience with either of these blood bank reagent vendors? Both exhibited at this year's AABB meeting. I believe that Diamed is out of Switzerland and Medion out of Germany. About two weeks ago I requested information from these companies but have thus far gotten no response. I was under the impression that their reagents were licensed in the U.S. but maybe I'm wrong. Thanks!

    Hi,

    Diamed is since last year BioRad and I think the reagents are not licensed in the US. Medion is a German/Switzerland Company and so far I know the cells are licensed in the U.S . In the next 3 month Biotest from Germany will come with their antiseras on the US market. Biotest sells over 50 years antisera in Germany and Europe and the quality of the products are very good. There is a Biotest office in the US

    Blood Bank Automation

    in Transfusion Services

    Posted

    I am interested in automation for a transfusion and blood bank service.

    We currently are tube typing.

    Does anyone use the Ortho Provue?

    Donna

    look after the Tango optimo !!it is an very userfriendly fully automated walk away system . If you need some more information come back to me

    :)

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