[Testing Rh prior 3 months post transfusion]

I am referring to Rh D typing

Thanks

[Testing Rh prior 3 months post transfusion]

I wonder if some people are testing or reporting Rh result prior to 3 months post transfusion.

[Group ABO reactions + and +s]

While in some cases when identifying antibodies it might be interesting to differentiate a regular reaction ex: 1+ than a strong reaction 1+s.

Do you see this importance for ABO-RH grouping or 1+ is sufficient ?

Thanks

[Mixed fields]

I would like to know if some people are grading mixed field 1 +, 2+ or if you are using only the terme mixed fields

Thanks

Why are you grading MF since this happens is some particular situations.

[Mixed fields]

I would like to know if some people are grading mixed field 1 +, 2+ or if you are using only the terme mixed fields

Thanks

[Agglutination reactions]

Thanks

[Agglutination reactions]

I would like to know if as a reference lab you are using a grade scale as the AABB recommandation such as 1+, 2+, 3+ and 4+ or if you you are using as well 1+½, 2+½ and 3+½ to help finding different andibody­.

If so how what are your descriptions for each of them.

Thanks

[double determination of phenotype]

Dumb Question from me here. Are we talking for immediate transfusion in a hospital....or permenant donor record in a donor center......or "this unit only" record in a donor center?

My question was originally for a donnor record ? dboutin

[double determination of phenotype]

Yes - it's the Arrêté du 26 Avril 2002 modifiant l'arrêté du 26 novembre 1999 relatif à la bonne exécution des analyses de biologie médicale, section 2.2.1-

"Une réalisation du phénotypage RH-KEL 1 comporte obligatoirement l'utilisation des réactifs anti-RH2, anti-RH3, anti-RH4, anti-RH5, anti-KEL 1 et du (des) réactif(s) témoin(s) adéquat(s). Il est recommandé d'utiliser des réactifs d'origine monoclonale.

..Sa définition est fonction des conditions techniques:

-si les opérations....sont strictement réalisées dans des conditions d'automation et d'informatisation décrites à l'article IV.....une détermination repose sur une seule réalisation executée à l'aide d'un lot de réactifs et par un technicien;

-dans tous les autres cas, une détermination repose sur deux réalisations executées pas deux techniciens différents. La saisie manuelle des résultats doit aussi passer par une double saisie effectuée par deux personnes différentes.

...Un phénotypage...valide est réalisé sur deux prélèvements différents à raison d'une détermination par prélèvement."

The other part of this document that is relevant is Annexe D III

"La validation analytique repose sur:

-résultats conformes des CQI;

-absence d'ambiguïté réactionelle avec chaque réactif;

.........

-absence de discordance entre deux réalisations;........."

Sorry this is all in French, it's a direct quote. In translation (for anyone who's interested) the relevant text is the decree of 26th April.....relative to the correct carrying out of biomedical analyses.

The carrying out of a phenotype for Rh and Kell has to include anti-C, c, E, e and K as well as adequate control reagents. It is recommended to use monoclonal reagents.

How the phenotype is carried out depends on the technical conditions:

If the phenotype, including all the operations relative to verification and registration of samples..are carried out according to the guidelines laid out in Article IV Automation and IT (this is a very long, complicated document that lays down strict guidelines for working with Automates - Anna) then the determination of a phenotype can be carried out on a single sample with one lot of reagents and by one single technician;

-in all other cases, a determination requires 2 determinations carried out by 2 different technicians. Manual reading must also be carried out by two different technicians.

A valid Rh/K phenotype is carried out on 2 different samples, with one test being carried out on each sample.

Then, from Annexe D III -

The validation of the test requires:

-correct Quality control results

-Clear positive or negative results with each reagent

......

-There must be no discrepancy between the two determinations.....

Phew!! Hope that helps

Thanks for your time and yes it helped a lot. dboutin

[double determination of phenotype]

In Australia, we phenotype donors on two separate donations before considering the phenotype confirmed. We follow the Code of Europe but, I have checked, and this requirement is not form the Code of Europe (12th ed).

I am not sure where we derived this practice from but I believe it to be necessary as mistakes in phenotyping do happen.

David

Thanks ! dboutin:)

[double determination of phenotype]

Thank you, Merci,

Savez-vous quelle norme ou réglement européen rend cette pratique obligatoire ?

Do you know which european standard or regulations that makes it mandatory ?

Thanks, Merci.

[double determination of phenotype]

I wonder if it is mandatory to make a phenotype determination twice on two separate draw in order to consider it confirmed.

Is somebody know ? If yes, which standard or regulation requires that ?

Thanks