We use the 2 and 2 rule.
The way I understood the math is that 3 and 3 rule = 0.05 p value if you're running only 6 cells total. We use a three cell screen and an 11 cell first-line panel. You should be able to get an acceptable p value based on those numbers of cells tested. On that note, there's a lot of discussion about the relevance of p value in the first place.
Kanter, M. H., Poole, G. D. and Garratty, G. (1997), Misinterpretation and misapplication of p values in antibody identification: the lack of value of a p value. Transfusion, 37: 816–822.
We use one homozygous cell for rule outs, with some exceptions.