We store autologous bone flaps at our hospital in blood bank in a -70 tissue freezer. Yesterday an auto bone flap was sent to surgery to be reimplanted. After the surgeon implanted the bone flap, the patient began having active intractible seizures. The surgeon removed the bone flap and it was returned to the blood bank. The bone flap was placed back into the -70 tissue freezer. The bone flap was cultured per our hospital procedure both when initially collected and after being removed this time. We have never had an auto bone flap that has been thawed returned to be refrozen. We would appreciate anyones thoughts on this situation.