bloodbankem

Thank you for the reply. The actual treatment of the cells is according to package insert. I'm not sure I'd classify preparing a reagent as a lab developed test (but I don't have any experience with what's classified as such). As far as QC goes, the insert just says "run a positive and negative control". Glycine max has been a quick means of QC with the ficin panel and would serve as the positive control, then just run 6% albumin as the negative control. I felt we should run an RH and FY system antibody, but that turned into a discussion of human source (availability) vs commercial (cost). Do you use commercial anti-M? And are you testing just pre-treatment (positive control) and post-treatment (negative control)?

Hello, We recently switched from Immucor's ficin-10 panel to Biorad's papain-treated panel. We'd like to continue using glycine max as our means of evaluating enzyme treatment of our autologous cells and/or any selected cells. Since no one manufactures lectin panels anymore, we're looking at purchasing it from Sigma and preparing our own stock solution. Does anyone have, and willing to share, their process for preparing and standardizing its use? Any other advice/comments on evaluating enzyme treatment? Thank you!