sunan20090128

Of the world's most populous nation - China. From Guangdong Zhongshan Blood Center, located in southern China, Guangdong Province is the province with the highest GDP in China.

Can change the order in gel card test? Why? When using gel card (anti-human globulin) for cross-matching , can be added by first serum , then, added red blood cells ?

Can not centrifuge regular(often) gel cards. May reduce test sensitivity. 搜索

I come from the world's most populous (first) country, please try to guess.

:cries: Introducing a new antigen-antibody reaction enhancer : SAN enhancer (SANE) After 20 years of hard work, I have invented a new kind of antigen-antibody reaction enhancer - SAN enhancer (SANE), it can be in the gel cards and the application of test tube method. SANE in the gel card, also known as "micro-gel enhancer" (Micro-column gel enhancer, MGE); SANE law in a test tube, also known as "test-tube method enhancer" (Test-tube method enhancer, TME ). SANE in the test tube method in blood group serology testing, instead of an indirect anti-human globulin test(IAT). After incubated in one minute can detect of red cell blood group IgG antibodies. The following are the specific steps 一、[MGE method steps] - gel card applications 50ul 1% of the red blood cell saline solution + 50ul serum / plasma + 50ul MGE, 37 ℃ incubated for 1 minute, centrifuged (900rpm, 2min, and then 2300rpm ,3-4min. Or 2000rpm, 5min.), The naked eye or equipment to read the results. Red blood cells sink to the bottom of the micro-column gel card negative reaction, if the red blood cells in the micro-column gel card or the top of the upper part of the positive reaction. Disadvantages: At present, MGE method can not detect a large number of 0.9% NaCl containing serum samples, as a pure saline can lead to false-positive reaction, so MGE method can not be used 0.9% NaCl as diluent for antibody titer test. 二、[TME method steps] - Application of test-tube method 50ul of 3% of the red blood cell saline solution +100 ul of serum / plasma + 100ul TME, 37 ℃ incubated for 1 minute, centrifuged (3100rpm, 15s; or 1000rpm, 1min.), The naked eye to read the results. Erythrocyte agglutination there is a positive reaction, whereas no agglutination for the negative. Benefits: TME can be used for serum, plasma, saline test, 0.9% NaCl will not lead to false-positive reaction, it can be used for detection of antibody titer, For example, determination of pregnant woman blood serum IgG anti-A ( titre . TME has been completed Drug Registrationin a country FDA ( TME has obtained a national FDA the drugs registration form). TME-related cross-matching (compatibility test) kit will be supplied in a country of the Asian marketin November 2009. Would welcome further research cooperation. SUN Ai-nong (Director, Professor) E-mail: sunainong@21cn.com

GP.Mur (Mi. Ⅲ) can be called Chinese blood do? Prepared AB-type anti-Mur standard serum. Recently, we found one cases of AB-type blood donors, serum containing anti-Mur, therefore, carried out a number of plasma exchange to prepare a batch of anti-Mur standard serum. The anti-Mur is very valuable because the AB-type non-ABO antibodies in human serum, so it can be used as determining human red blood cell Mur antigen'santibody reagents. It can be used as populations red blood cell GP.Mur (Mi. Ⅲ) phenotype frequency of the investigation. The cases of IgM anti-Mur has passed the international authentication. Anti-Mur is an important Miltenberger blood group system antibodies, our preliminary experiments showed that in southern China, anti-Mur frequency of 0.11% (15/14210), red blood cell Mur antigen frequency of 6-7% . Anti-Mur is the world's largest number of irregular antibodies in the Chinese. Question 1: GP.Mur (Mi. Ⅲ) can be called Chinese blood do? The general international transfusion medicine community, said Miltenberger blood group antigens and antibodies for the "low frequency", it seems that the Chinese are not the low-frequency, because the population frequency of 6-7% GP.Mur positive close to the AB-type ABO blood group frequencies. So, name given GP.Mur (Mi. Ⅲ) phenotype of the Chinese red blood cell, as if some sense. Question 2: Why is China Mur antigen-positive blood donors in north and south such a big difference in the rate? In southern China, red blood cell Mur antigen (GP.Mur) positive 6-7%. However, in northern China, such as the Shanghai blood donors Mur antigen positive rate 0.5% (15/2970). What has caused the North and the South China Mur antigen-positive blood donors in such a big difference in the rate, who can in theory explain? Thanks Would welcome further research cooperation. SUN Ai-nong (Director, Professor) E-mail: sunainong@21cn.com

Looking for Cooperation of developing Micro-column gel enhancer （MGE） I have invented a product called micro-column gel enhancer (MGE). The purpose of it is to shorten the incubation times of Gel Coombs from 15 minutes to 1 minute, MGE can also improve the sensitivity of IgG antibody detection. Is anybody interested in such co-operation? Mr. Sun AN (Professor). E-mail:sunainong@21cn.com