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Showing results for tags 'manual solid phase'.
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Hi Everyone, I am new to this site, but could not find any threads specifically pertaining to some of the peculiar solid phase issues my lab seems to deal with. We have one Echo, one Galileo, and a solid phase station. We have been using them for a number of years. Like many people have posted on the forum, there seems to be some strong panagglutiniation “false positives” but ours only appear on the machines. Interestingly, if we run these samples in manual solid phase (MSP), both the screens and panel cells will be negative (or show an underlying antibody). Warm autos are then ruled out. If it is still a panagglutinin in MSP, we move to gel and follow through with warm auto testing. Though MSP is supposed to be a little less sensitive than the machines, a 3+ does would not normally turn negative. I am posting to see if anyone has the same experience, where machine solid phase testing is all positive and manual solid phase testing is negative. This leads me to believe that the “solid phase antibody is actually an antibody to the pre-embedded screen and panel strips because there is no other difference between the two testing methods. Does any one else go to manual solid phase before going to their secondary method?
