Hi All

Lewis antibody exclusions.

Realise they are rarely clinically significant only causing HTR and would perform crossmatch compatible. However I want to clarify the theory for exclusions.  

Human Anti-Lea is found in Le(a-b-) however exception to rule in Tawain where 10 Lea (a-b+) patients with anti-Lea were found. Most anti-Lea contains weak anti- Leb causing false positives.

However monoclonal reagents seem to indicate no cross reactivity? What reagents do you use to exclude the presence of either anti-Lea or Leb ? 

Thanks

the same as you would for any other antibody

But you need to make sure that if there is an anti-Lea or -Leb present that it is reacting strictly at 37°C.  Otherwise no clinical relevance at all