Hello

What are the pros and cons of using IgG vs AHG  when performing a tube crossmatch or tube screen assuming that the tube method is a back up method to MTS gel.

Is it possible to miss a complement binding IgM antibody early on by using IgG only. The standards seem to indicate that IgG only is required. 

Thanks.

Richard

 

It is possible, but highly improbable.

On 6/22/2018 at 3:22 PM, richj said:

Hello

What are the pros and cons of using IgG vs AHG  when performing a tube crossmatch or tube screen assuming that the tube method is a back up method to MTS gel.

Is it possible to miss a complement binding IgM antibody early on by using IgG only. The standards seem to indicate that IgG only is required. 

Thanks.

Richard

Does the MTS gel card you typically use contain polyspecific antiglobulin reagent (anti-IgG + anti-complement)  or does it just contain anti-IgG ? I think most users are using anti-IgG cards, and if that is the case, they're already dealing with the "Is it possible to miss a complement binding IgM antibody early on by using IgG only." issue.

Poly Specific AHG is less expensive.

 

You can avoid some annoying clinically insignificant cold reactive antibodies by using anti-IgG.

Stopped using Polyspecific AHG in lieu of Monospecific Anti-IgG for antibody screen/crossmatch tube testing in 1980 and of course Anti-IgG Gel testing since 1996.  No occurrence since then that would cause me to reverse those decisions.