I am trying to find out what everyone uses for their QC on the Gel Card antibody screens. We do not use a kit.  In the past we used dilute Anti-D and Dilute Anti-c.  I have taken over the manager position and was reviewing the procedures and noticed a notation in the QC procedure stating to never use Rh anti-sera for control purposes for Gel cards.  They switched to Jka and Jkb which can be a little wonky at times and also expensive. I can't find anything anywhere that mentions not using the RH sera.  I may have missed something in my absence but would like to switch back until we get our automation in place.

 

Thanks in advance for your responses

I think because Rh antisera is usually not IgG  and will agglutinate with cells in buffer cards so it is really not QCing the IgG activity in your IgG card.  

Edited April 19, 20187 yr by R1R2

Good point about some antigen typing antisera not appropriate for gel QC.  

For our QC, we do indeed use a kit that is appropriate for gel.  We dilute down the anti-sera from the Ortho Confidence QC system (same one that we use for ABO reagent QC), which has anti-D and anti-c IgG. 

Scott

I use Immucor's CorQc kit for my positive controls.  anti-D,-c and the cells are AB Pos.  dilute the ab 1:10.