with buying the new screen cells or changing in lot numbers of vials of antibody detection in the blood bank, we need to evaluate them for appropriate quality. how do you perform this?

In the US, at a minimum, you must follow the manufacturer's instructions regarding QC and any other required validation.  We run QC material on all reagents once a day whether there is a lot change or not.  When a new lot number is put into use, it is QC'd first before using for patients. 

We do NOT do any sort of lot-to-lot correlation.

Scott

we use a dilute anti-D with a 2 cell screen.

 

We run QC once per day with known positive reagent antisera. If you’re referring to lot to lot comparison, we do not currently do that, but you could use a patient sample with a known positive screen or a neg sample spiked with a couple drops of anti D, as suggested above. 

USA here ...

As for all our reagents, we test the lot when it arrives using the same protocol as the daily QC (pos + neg) to assure that it arrived satisfactory.

This way, there are no surprises on the day we discard the old lot and start the new one.

We dilute up some anti-D and use an aliquot of that to crosscheck the newly received antibody screening cells with the screening cells currently in use.  When the cells are put into use, we check all three cells using anti-D or anti-c diluted up so that we are testing all cells for something positive.

for us here,, for the antibody ID we use CCC + Anti-D diluted 1:256 at least for every lot that come in

Edited January 27, 20196 yr by radwan1411

We use a commercial QC kit. The positive control is a blend of Rh antibodies that will give a positive reaction with all three screen cells. You could make something like that by spiking a negative plasma sample with anti-D and anti-c. For a negative control, we use a patient sample that was testing in the previous 2-3 days and was found to be non-reactive (negative antibody screen).

Beware of using reagent antisera to do your QC.  The buffers in the reagents can interact with the buffers in the cells and, if using anything other than tube, the buffers in the support medium.  You may get false positive results