Validating a new COBE for a 1-liter RBC wash and a 2-liter RBC wash.  When it comes to the PQ, we are setting our expected results and are struggling on how to prove the wash worked as expected.  Things like how much volume was lost and the amount of red cells we are able to recover are our main two parameters currently.  Should we look into protein levels of the blood before and after the wash to ensure the RBC was adequately washed?

Any help and guidance will be much appreciated!

We don't even do voluome and H&H anymore. We do pre and post protein and do a percent removal. > 90% acceptable.