Good Morning!!

 

Looking for some insight from blood bankers........ I have noticed that procedures vary by institutions for the prewarm technique. Some facilities use potentiators (PEG, LISS) with the prewarm method, some do not use any potentiators for the prewarm method. The Technical Manual does not say either way.

 

Any thoughts??

Thanks,

 

Ficin

 

NHSBT Reference Laboratories in the UK use LISS with the pre-warming technique, but, just occasionally, if this does not "get rid" of the "cold" antibody, we will use NISS.

One can use any of the manual tube techniques, as long as one is comparing apples to apples - if one's basic (normal) test uses a potentiator, one must use it in the pre-warmed verison. As Malcolm suggests, sometimes a plain vanilla saline antiglobulin test is the way to go, provided the "cold" reacts in that phase.

Edited April 10, 20178 yr by exlimey
Typo/clarification

I try to avoid using it if at all possible.

I routinely used PeG in tube testing so I used when using prewarmed techniques.

In general, we do not use potentiators if we go to a prewarm.

Scott

We do not use potentiators during a prewarmm.

 

On 4/10/2017 at 11:03 AM, SMILLER said:

In general, we do not use potentiators if we go to a prewarm.

Scott

 

9 hours ago, Sherie Bruns said:

We do not use potentiators during a prewarmm.

Presumably the operators check that the troublesome reactivity that they are trying to circumvent is actually present in the unpotentiated test ?

we do not use potentiator with prewarm...

We do use potentiator with the prewarm.  We also use it when testing our WAHA patients.  I know there are many labs who do not use it for WAHA workups.   I was told that in the end the potentiator really only reduced your incubation time.  If this is incorrect someone please enlighten me

We do not use potentiator with a prewarm. We incubate for 15 separate then 30 together.