When testing DTT-treated K+ and K- cells for QC, should you use AHG anti-K, monoclonal anti-K or does it matter?

I don't know for certain, but the point is that dithiothreitol is a reducing agent that will break disulphide bonds.  The Kell glycoprotein is highly folded due to many disulphide bonds from the 15 cysteine residues in the molecule.  It is the breaking of these disulphide bonds that prevents expression of the Kell Blood Group System antigens, as the glycoprotein loses its tertiary structure.

It would seem logical, therefore, that either AHG anti-K or Monoclonal anti-K could be used, but, I stress again, I am not certain.

11 hours ago, jollymon said:

When testing DTT-treated K+ and K- cells for QC, should you use AHG anti-K, monoclonal anti-K or does it matter?

Why would you treat/test K= rbcs?  You want to see if the K ag is being destroyed so your untreated K+ cell is your positive ct, the treated cell should be negative.

Test a K+ or k+ DTT treated cell by your normal method, if you have a choice use the quickest method.

Thank you all for your replies!!  Very helpful!